Long‐term storage at −80°C of hematopoietic progenitor cells with 5‐percent dimethyl sulfoxide as the sole cryoprotectant

@inproceedings{Galms1999LongtermSA,
  title={Long‐term storage at −80°C of hematopoietic progenitor cells with 5‐percent dimethyl sulfoxide as the sole cryoprotectant},
  author={Antonio Galm{\'e}s and J. Besalduch and J Bargay and Andr{\'e}s Novo and Miguel Morey and Jos{\'e} M. Guerra and Mar{\'i}a Antonia Dur{\'a}n},
  year={1999}
}
BACKGROUND: Hematopoietic progenitor cells (HPCs) can be cryopreserved and stored below −120°C in liquid nitrogen or at −80°C in mechanical freezers. STUDY DESIGN AND METHODS: The feasibility of long-term storage of HPCs at −80°C was investigated. The studies included a comparison of 5- and 10-percent dimethyl sulfoxide (DMSO) as cryoprotectant at various lengths of storage time. Mononuclear cell (MNC) recovery and viability and colony-forming unit-granulocytemacrophage (CFU–GM) and… CONTINUE READING

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