Long‐term expanding human airway organoids for disease modeling

  title={Long‐term expanding human airway organoids for disease modeling},
  author={Norman Sachs and Angelos Papaspyropoulos and Domenique D Zomer-van Ommen and Inha Heo and L. B{\"o}ttinger and Dymph Klay and F. Weeber and Guizela Huelsz-Prince and Nino Iakobachvili and G. D. Amatngalim and Joep de Ligt and A. van Hoeck and N. Proost and M. Viveen and A. Lyubimova and Luc Teeven and Sepideh Derakhshan and Jeroen Korving and H. Begthel and J. F. Dekkers and K. Kumawat and Emilio Ramos and M. V. van Oosterhout and G. Offerhaus and D. Wiener and E. P. Olimpio and K. Dijkstra and E. Smit and M. van der Linden and S. Jaksani and M. van de Ven and J. Jonkers and Anne C. Rios and E. Voest and C. V. van Moorsel and C. K. van der Ent and E. Cuppen and A. van Oudenaarden and F. Coenjaerts and L. Meyaard and L. Bont and P. Peters and S. Tans and J. V. van Zon and S. Boj and R. Vries and J. Beekman and H. Clevers},
  journal={The EMBO Journal},
  • Norman Sachs, Angelos Papaspyropoulos, +45 authors H. Clevers
  • Published 2019
  • Biology, Medicine
  • The EMBO Journal
  • Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow… CONTINUE READING
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