Locked nucleic acids in PCR primers increase sensitivity and performance.

  title={Locked nucleic acids in PCR primers increase sensitivity and performance.},
  author={Kaye N. Ballantyne and Roland A. H. van Oorschot and R. J. Mitchell},
  volume={91 3},
The incorporation of locked nucleic acids (LNAs) into oligonucleotide primers has been shown to increase template binding strength and specificity for DNA amplification. Real-time PCR and DNA sequencing have been shown to be significantly enhanced by the use of LNAs. Theoretically, increasing primers' binding strength may also increase the sensitivity of conventional PCR, reducing minimum template requirements. We compared LNA-modified PCR primers with their standard DNA counterparts for… CONTINUE READING


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