Locked nucleic acids (LNAs) reveal sequence requirements and kinetics of Xist RNA localization to the X chromosome.

@article{Sarma2010LockedNA,
  title={Locked nucleic acids (LNAs) reveal sequence requirements and kinetics of Xist RNA localization to the X chromosome.},
  author={Kavitha Sarma and Pierre Levasseur and Alexander Aristarkhov and Jeannie T Lee},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2010},
  volume={107 51},
  pages={22196-201}
}
A large fraction of the mammalian genome is transcribed into long noncoding RNAs. The RNAs remain largely uncharacterized as the field awaits new technologies to aid functional analysis. Here, we describe a unique use of locked nucleic acids (LNAs) for studying nuclear long noncoding RNA, an RNA subclass that has been less amenable to traditional knockdown techniques. We target LNAs at Xist RNA and show displacement from the X chromosome with fast kinetics. Xist transcript stability is not… CONTINUE READING

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