Location of the aromatic binding site and preparation of an aromatic derivative of glycogen phosphorylase.


Rabbit muscle glycogen phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-D-glucosyltransferase, EC was inactivated by 1,5-difluoro-2,4-dinitrobenzene at pH 7.6 at a rate much faster than by 1-fluoro-2,4-dinitrobenzene. The reaction was very specific at low concentration of 1,5-difluoro-2,4-dinitrobenzene. Glucose 1-phosphate, glucose 6… (More)


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