Location of smooth-muscle myosin and tropomyosin binding sites in the C-terminal 288 residues of human caldesmon.

@article{Huber1995LocationOS,
  title={Location of smooth-muscle myosin and tropomyosin binding sites in the C-terminal 288 residues of human caldesmon.},
  author={Pia A J Huber and Iain D Fraser and Steven Marston},
  journal={The Biochemical journal},
  year={1995},
  volume={312 ( Pt 2)},
  pages={617-25}
}
We have produced nine recombinant fragments, H1 to H9, from a human cDNA that codes for the C-terminal 288 residues of caldesmon. The fragment H1, encompassing the 288 residues, is equivalent to domains 3 and 4 of caldesmon (amino acids 506-793 in human, 476-737 in the chicken gizzard sequence). It has been shown [Huber, Redwood, Avent, Tanner and Marston (1993) J. Muscle Res. Cell Motil. 14, 385-391] to bind to actin, Ca(2+)-calmodulin, tropomyosin and myosin. The fragments, H2 to H9, differ… CONTINUE READING

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