Location and functions of Inebriated in the Drosophila eye

  title={Location and functions of Inebriated in the Drosophila eye},
  author={Janusz Borycz and Anna B. Ziegler and Jolanta A. Borycz and Guido Uhlenbrock and Daniel Tapken and Lucía Cáceres and Michael Hollmann and Bernhard T. Hovemann and Ian A. Meinertzhagen},
  journal={Biology Open},
ABSTRACT Histamine (HA) is a neurotransmitter in arthropod photoreceptors. It is recycled via conjugation to β-alanine to form β-alanylhistamine (carcinine). Conjugation occurs in epithelial glia that surround photoreceptor terminals in the first optic neuropil, and carcinine (CA) is then transported back to photoreceptors and cleaved to liberate HA and β-alanine. The gene Inebriated (Ine) encodes an Na+/Cl−-dependent SLC6 family transporter translated as two protein isoforms, long (P1) and… 


tan and ebony Genes Regulate a Novel Pathway for Transmitter Metabolism at Fly Photoreceptor Terminals
It is suggested that reversible synthesis of carcinine occurs in surrounding glia, serving to trap histamine after its release at photoreceptor synapses; subsequent hydrolysis liberates histamine for reuptake.
The metabolism of histamine in the Drosophila optic lobe involves an ommatidial pathway: β-alanine recycles through the retina
A novel function for pigment cells, which not only screen ommatidia from stray light but also store and transport β-alanine and carcinine is demonstrated, consistent with a β-Alanine-dependent histamine recycling pathway occurring not only in the photoreceptor terminals in the lamina neuropile, but vertically via a long pathway that involves the retina.
Histamine: A Neurotransmitter Candidate for Drosophila Photoreceptors
  • P. Sarthy
  • Biology, Medicine
    Journal of neurochemistry
  • 1991
Observations strongly suggest that histamine is a major neurotransmitter used by Drosophila photoreceptors.
Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors
It is revealed that CarT is required for histamine recycling at histaminergic photoreceptors and evidence for a CarT-dependent neurotransmitter trafficking pathway between glial cells and photoreceptor terminals is provided.
Alternative Tasks of Drosophila Tan in Neurotransmitter Recycling Versus Cuticle Sclerotization Disclosed by Kinetic Properties*
Kinetic parameters of Tan reveal characteristic differences in Km and kcat values of carcinine and β-alanyl-dopamine cleavage, which conclusively illustrate the divergent tasks met by Tan.
The Role of Carcinine in Signaling at the Drosophila Photoreceptor Synapse
Pharmacological and genetic epistatic evidence that ine encodes a carcinine neurotransmitter transporter is provided and it is speculated that the oscillations observed in mutant ine ERG traces are the result of the aberrant activity of a putative H3 receptor.
Drosophila ABC transporter mutants white, brown and scarlet have altered contents and distribution of biogenic amines in the brain
The results are consistent with the proposal that histamine uptake by the epithelial glia may be white dependent, and Behavioral abnormalities in white, brown and scarlet mutants could arise because aminergic neurons in the Drosophila brain have reduced amine for release.
The dynamics of signaling at the histaminergic photoreceptor synapse of arthropods
This work has shown that a major pathway in Drosophila, and possibly other arthropod species, is by conjugation of histamine to beta-alanine to form carcinine in adjacent glia, which returns to the photoreceptors where it is hydrolysed to liberate histamine, which is then loaded into synaptic vesicles.
The determination of histamine in the Drosophila head
A high performance liquid chromatography method with pre-column o-phtaldialdehyde-mercaptoethanol (OPA-ME) derivatization and electrochemical detection enables the fast and accurate measurement of histamine in the heads of Drosophila, suitable for screening mutants.
A neurotransmitter transporter encoded by the Drosophila inebriated gene.
The cloning and sequence analysis of ine concludes that ine mutations cause increased excitability of the Drosophila motor neuron by causing the defective reuptake of the substrate neurotransmitter of the ine transporter and thus overstimulation of the motor neurons by this neurotransmitter.