Localizing Protein in 3D Neural Stem Cell Culture: a Hybrid Visualization Methodology

Abstract

The importance of 3-dimensional (3D) topography in influencing neural stem and progenitor cell (NPC) phenotype is widely acknowledged yet challenging to study. When dissociated from embryonic or post-natal brain, single NPCs will proliferate in suspension to form neurospheres. Daughter cells within these cultures spontaneously adopt distinct developmental… (More)
DOI: 10.3791/2483

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