Localized transfection with magnetic beads coated with PCR products and other nucleic acids

@article{Santori2006LocalizedTW,
  title={Localized transfection with magnetic beads coated with PCR products and other nucleic acids},
  author={Maria Isabel Santori and Cayetano Gonzalez and Luis Serrano and Mark Isalan},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={526-531}
}
The bead transfection method involves binding nucleic acids onto 3-μm-diameter paramagnetic beads, treating the beads with transfection reagent, and using them as scaffolds to direct transfection to individual cells or regions in a population. Typically, PCR products are used because they can be conveniently generated using biotinylated primers and can introduce site-directed mutations, without the need for cloning or plasmid purification. However, the method can be adapted to transfect plasmid… 

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  • A. SasakiM. Kinjo
  • Biology
    Journal of controlled release : official journal of the Controlled Release Society
  • 2010

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