Localization to the proteasome is sufficient for degradation.

  title={Localization to the proteasome is sufficient for degradation.},
  author={Daniel Maarten Janse and Bernat Crosas and Daniel Daniel Finley and George M. Church},
  journal={The Journal of biological chemistry},
  volume={279 20},
The majority of unstable proteins in eukaryotic cells are targeted for degradation through the ubiquitin-proteasome pathway. Substrates for degradation are recognized by the E1, E2, and E3 ubiquitin conjugation machinery and tagged with polyubiquitin chains, which are thought to promote the proteolytic process through their binding with the proteasome. We describe a method to bypass the ubiquitination step artificially both in vivo and in a purified in vitro system. Seven proteasome subunits… CONTINUE READING
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Methods Enzymol

  • U. Baron, H. Bujard
  • 2000

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