Localization of the thyroglobulin gene by in situ hybridization to human chromosomes

  title={Localization of the thyroglobulin gene by in situ hybridization to human chromosomes},
  author={J L Berg{\'e}-lefranc and Guy Cartouzou and Marie Genevi{\`e}ve Mattei and Edith Passage and Christiane Malezet-Desmoulins and Serge Lissitzky},
  journal={Human Genetics},
SummaryThe human thyroglobulin gene was mapped by in situ hybridization whereby a 3H-labeled recombinant plasmid DNA containing a fragment of 2.3 kilobases of human thyroglobulin gene was hybridized to human chromosome preparations. A high proportion (25%) of hybridized metaphases exhibited silver grains at the distal portion of the long arm of chromosome 8. Analysis of the grain position at this site indicated that the chromosomal localization of the human thyroglobulin gene was 8q242-8q243. 
DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome
Recombinant plasmid clone B74 containing a human single-copy DNA segment of 6 kilobases (kb) was localized by in situ hybridization on band p113 of chromosome 18 to identify precisely a small supernumerary chromosome as an isochromosome i(18p).
Localization by in situ hybridization of the coagulation factor IX gene and of two polymorphic DNA probes with respect to the fragile X site
SummaryThe coagulation factor IX gene and two other polymorphic loci corresponding to DNA probes 52 A and St 14 have been previously localized in the q27 to qter region of the human X chromosome. In
Molecular advances in thyroglobulin disorders.
  • C. Rivolta, H. Targovnik
  • Biology, Medicine
    Clinica chimica acta; international journal of clinical chemistry
  • 2006
Hydrolase, theEnzymeDeficient in Hereditary Tyrosinemia: Assignment oftheGeneto Chromosome15
The isolation of humanFAHcDNA clones indicates that the single polypeptide chain encoded by theFAHgenecontains all thegenetic information required for functional activity, suggesting that the dimer found Invivoisahomodimer.
New insights in the human thyroglobulin structure
All reportedd TG mutations causing dyshormonogenesi s in man and animal are designate dd in the nucleotide and amino acid sequences.
Gene mapping and medical genetics Human chromosome 8
The 27 genes assigned to chromosome 8 at the Ninth Human Gene Mapping Workshop thus represent a third of the expected number, and in six loci corresponding to fragile sites, three pseudogenes, and four gene-like sequences were is a of the 500 to 5000 gene loci expected from a genome that 10 000 and 100 000.
Integrity of the thyroglobulin locus in tricho-rhino-phalangeal syndrome II
The results indicate that the locus of the thyroglobulin gene is intact in the patient with an interstitial deletion of proximal band 8q24, which confirms its more distal localization reported earlier by Bergé-Lefranc et al. (1985).
De novo complex autosomal translocation involving chromosomes 8, 13 and 15 in a girl with a sporadic retinoblastoma
A 5-month-old female with sporadic monolateral retinoblastoma with a constitutional de novo complex autosomal translocation involving chromosomes 8, 13 and 15 resulting in a deletion of chromosome 13q14 confirmed by esterase D assay is reported.
Comparative map for mice and humans
This report summarizes the status of this comparative map and provided a listing of homologous genes and anonymous loci that have been mapped in mice and humans together with references documenting homology and the chromosomal and linkage assignments.
Naturally occurring mutations in the thyroglobulin gene.
Molecular analyses indicate that at least some of these alterations in the Tg gene result in a secretory defect and an endoplasmic reticulum storage disease (ERSD).


Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization
In situ hybridization to human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling of band p36 of chromosome 1, such that 22% of all chromosome grains were located on this region.
Localization of the human insulin gene to the distal end of the short arm of chromosome 11.
The results demonstrate chromosomal localization of the human insulin gene to 11p15 and a significant percentage of hybridized cells exhibited silver grains on the distal end of the short arm (band p15) of chromosome 11.
High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.
It is shown that the resolution of in situ hybridization can be increased by hybridizing the probe to stretched prometaphase chromosomes with high-resolution banding obtained after 5-bromodeoxyuridine treatment of the cells and with a Hoechst 33258/Giemsa chromosome-staining method.
The human thyroglobulin gene contains two 15-17 kb introns near its 3'-end.
Cloned overlapping segments of the human thyroglobulin gene are cloned from a genomic cosmid library and blot hybridization under non-stringent conditions shows the presence of only one copy of this gene in the human genome and the absence of other closely related sequences.
Localization of a unique gene by direct hybridization in situ.
A general technique for the localization of a unique gene, which requires no prior chromosome fractionation, is developed and shown that, with an autoradiographic exposure time of only 12 days, use of this technique correctly localized the human alpha-globin gene cluster to chromosome 16.
Localization of human immunoglobulin kappa light chain variable region genes to the short arm of chromosome 2 by in situ hybridization.
The results show that human kappa light chain genes are located in the region of the break point observed in specific chromosomal translocations associated with Burkitt lymphoma.
Cloning of four DNA fragments complementary to human thyroglobulin messenger RNA.
Human thyroglobulin mRNA was isolated from Graves' goitres by size selection of total poly(A)-rich RNA in a sucrose gradient and showed a single component of approximately 8500 bases by gel electrophoresis.
Two human c-onc genes are located on the long arm of chromosome 8.
The results suggest that translocations of the human c-mos or c-myc genes may be causally related to neoplastic transformation.
Structural organization of the 3' half of the rat thyroglobulin gene.
The structural organization of an 80 Kb segment of rat DNA, which encodes for about 40% of Thyroglobulin mRNA at the 3' end, is reported, and the minimum length of the rat TGB gene will be 150 Kb, which makes this gene the largest so far identified eukaryotic gene.