Localization of the guinea pig eosinophil major basic protein to the core of the granule

@article{Lewis1978LocalizationOT,
  title={Localization of the guinea pig eosinophil major basic protein to the core of the granule},
  author={David M Lewis and Jon Lewis and David A. Loegering and Gerald J. Gleich},
  journal={The Journal of Cell Biology},
  year={1978},
  volume={77},
  pages={702 - 713}
}
The localization of the guinea pig eosinophil major basic protein (MBP) within the cell was investigated by the use of immunoelectron microscopy and by isolation of the granule crystalloids. First, by immunoperoxidase electron microscopy, we found that the MBP of eosinophil granules is contained within the crystalloid core of the granule. Specific staining of cores was present when rabbit antiserum to MBP was used as the first stage antibody in a double antibody staining procedure, whereas… CONTINUE READING