Localization of the essential histidine and carboxylate group in D-xylose isomerases.

Abstract

D-Xylose isomerases from different bacterial strains were chemically modified with histidine and carboxylate-specific reagents. The active-site residues were identified by amino acid sequence analysis of peptides recognized by differential peptide mapping on ligand-protected and unprotected derivatized enzyme. Both types of modified residues were found to… (More)

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@article{Vangrysperre1990LocalizationOT, title={Localization of the essential histidine and carboxylate group in D-xylose isomerases.}, author={W A Vangrysperre and J R Van Damme and Jo{\"e}l Vandekerckhove and C K. De Bruyne and R. Cornelis and H L Kersters-Hilderson}, journal={The Biochemical journal}, year={1990}, volume={265 3}, pages={699-705} }