Localization of the binding site of tissue-type plasminogen activator to fibrin.

  title={Localization of the binding site of tissue-type plasminogen activator to fibrin.},
  author={Akitada Ichinose and Koji Takio and Kazuo Fujikawa},
  journal={The Journal of clinical investigation},
  volume={78 1},
Functionally active A and B chains were separated from a two-chain form of recombinant tissue-type plasminogen activator after mild reduction and alkylation. The A chain was found to be responsible for the binding to lysine-Sepharose or fibrin and the B chain contained the catalytic activity of tissue-type plasminogen activator. An extensive reduction of two-chain tissue-type plasminogen activator, however, destroyed both the binding and catalytic activities. A thermolytic fragment, Fr. 1, of… CONTINUE READING

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