Localization of the binding site of tissue-type plasminogen activator to fibrin.

@article{Ichinose1986LocalizationOT,
  title={Localization of the binding site of tissue-type plasminogen activator to fibrin.},
  author={Akitada Ichinose and Koji Takio and Kazuo Fujikawa},
  journal={The Journal of clinical investigation},
  year={1986},
  volume={78 1},
  pages={163-9}
}
Functionally active A and B chains were separated from a two-chain form of recombinant tissue-type plasminogen activator after mild reduction and alkylation. The A chain was found to be responsible for the binding to lysine-Sepharose or fibrin and the B chain contained the catalytic activity of tissue-type plasminogen activator. An extensive reduction of two-chain tissue-type plasminogen activator, however, destroyed both the binding and catalytic activities. A thermolytic fragment, Fr. 1, of… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 27 references

Amino acid sequence of the heavy chain of human a - Factor XIIa ( activated Hageman Factor )

  • M. Nishida H. Arimura, T. Suyama
  • J . Biol . Chem .
  • 1985

Simultaneous selectivity optimization of mobile and stationary phases in reversed - phase liquid chromatography for iso - cratic separations of phenylthiohydantoin amino acid derivatives

  • R. Granberg
  • J . Chromatogr .
  • 1985

teolytic cleavage of single - chain pro - urokinase induces conformational change which follows activation of the zymogen and reduction of its high affinity for fibrin

  • A. Ichinose, K. Fujikawa
  • J . Biol . Chem .
  • 1985

Proteolytic activation of tissue plasminogen activator by plasma and tissue enzymes

  • N. Bergsdorf M. Kallstrom, P. Wallen
  • Fed . Eur . Biochem . Soc . Lett .
  • 1984

The structure of the human tissue - type plasminogen activator gene : correlation of intron and exon structures to functional and structural domains

  • L. Sottrup-Jensen, H. Claeys, M. Zajdel, T. E. Petersen
  • Proc . NatL Acad . Sci . USA .
  • 1984

Cloning and expressiorn of human tissue - type plasminogen activator cDNA in E . coli

  • F. Elgh
  • Nature ( Lond . ) .
  • 1983

Immunological characterization of plasminogen activators in the human vessel wall

  • J. Grondahl-Hansen, P. A. Andreasen, K. Dano
  • J . Clin . Pathol .
  • 1983

Similar Papers

Loading similar papers…