Localization of the active site of type II dehydroquinases. Identification of a common arginine-containing motif in the two classes of dehydroquinases.

@article{Krell1996LocalizationOT,
  title={Localization of the active site of type II dehydroquinases. Identification of a common arginine-containing motif in the two classes of dehydroquinases.},
  author={Tino Krell and Malcolm James Horsburgh and Alan H Cooper and Sharon M Kelly and John R. Coggins},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 40},
  pages={24492-7}
}
A novel method based on electrospray mass spectrometry (Krell, T., Pitt, A. R., and Coggins, J. R. (1995) FEBS Lett. 360, 93-96) has been used to localize active site residues in the type I and type II dehydroquinases. Both enzymes have essential hyper-reactive arginine residues, and the type II enzymes have an essential tyrosine residue. The essential hyper-reactive Arg-23 of the Streptomyces coelicolor type II enzyme has been replaced by lysine, glutamine, and alanine residues. The mutant… CONTINUE READING