Localization of the Q beta replicase recognition site in MDV-1 RNA.

@article{Nishihara1983LocalizationOT,
  title={Localization of the Q beta replicase recognition site in MDV-1 RNA.},
  author={Tsutomu Nishihara and David R. Mills and Fred Russell Kramer},
  journal={Journal of biochemistry},
  year={1983},
  volume={93 3},
  pages={669-74}
}
Fragments of MDV-1 RNA (a small, naturally occurring template for Q beta replicase) that were missing nucleotides at either their 5' end or their 3' end were still able to form a complex with Q beta replicase. By assaying the binding ability of fragments of different length, it was established that the binding site for Q beta replicase is determined by nucleotide sequences that are located near the middle of MDV-1 RNA. Fragments missing nucleotides at their 5' end were able to serve as… CONTINUE READING