Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization

@article{Harper1981LocalizationOS,
  title={Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization},
  author={M. E. Harper and Grady F. Saunders},
  journal={Chromosoma},
  year={1981},
  volume={83},
  pages={431-439}
}
Recombinant lambda bacteriophage clone H3 containing a human DNA segment of 14.9 kb present in one or two copies per haploid genome was isolated. In situ hybridization to human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling (53% of cells) of band p36 of chromosome 1, such that 22% of all chromosomal grains were located on this region. Hybridization was dependent upon the presence of dextran sulfate in the hybridization mixture and was not affected by… CONTINUE READING

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