Localization of reactive cysteine residues by maleidoyl undecagold in the mitochondrial creatine kinase octamer.

@article{Schnyder1995LocalizationOR,
  title={Localization of reactive cysteine residues by maleidoyl undecagold in the mitochondrial creatine kinase octamer.},
  author={Thomas Schnyder and Peter Tittmann and Heiner Winkler and Heinz Gross and Theo Wallimann},
  journal={Journal of structural biology},
  year={1995},
  volume={114 3},
  pages={209-17}
}
Octamers of mitochondrial creatine kinase (Mi-CK) were modified with the thiol-specific reagents N-ethylmaleimide or the gold-coupled derivative, maleidoyl undecagold. The kinetics of inhibition of the Mi-CK catalysis was shown to be comparable for both reagents, suggesting that the large gold cluster complex is accessible to the reactive cysteines. SDS-PAGE analysis revealed that two of eight cysteines per Mi-CK monomer were labeled with maleidoyl undecagold with a similar affinity for the… CONTINUE READING