Localization of proteins bound to a replication origin of human DNA along the cell cycle.

@article{Abdurashidova2003LocalizationOP,
  title={Localization of proteins bound to a replication origin of human DNA along the cell cycle.},
  author={Gulnara Abdurashidova and Miltcho Bojanov Danailov and A. Ochem and Gianluca Triolo and Vera Djeliova and Sorina Radulescu and Alessandro Vindigni and Silvano Riva and A. Falaschi},
  journal={The EMBO journal},
  year={2003},
  volume={22 16},
  pages={4294-303}
}
The proteins bound in vivo at the human lamin B2 DNA replication origin and their precise sites of binding were investigated along the cell cycle utilizing two novel procedures based on immunoprecipitation following UV irradiation with a pulsed laser light source. In G(1), the pre-replicative complex contains CDC6, MCM3, ORC1 and ORC2 proteins; of these, the post-replicative complex in S phase contains only ORC2; in M phase none of them are bound. The precise nucleotide of binding was… CONTINUE READING

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