Localization of mitochondrial DNA encoded cytochrome c oxidase subunits I and II in rat pancreatic zymogen granules and pituitary growth hormone granules

  title={Localization of mitochondrial DNA encoded cytochrome c oxidase subunits I and II in rat pancreatic zymogen granules and pituitary growth hormone granules},
  author={Skanda K. Sadacharan and Bhag Singh and Timothy Bowes and Radhey S. Gupta},
  journal={Histochemistry and Cell Biology},
Cytochrome c oxidase (COX) complex is an integral part of the electron transport chain. Three subunits of this complex (COX I, COX II and COX III) are encoded by mitochondrial (mit-) DNA. High-resolution immunogold electron microscopy has been used to study the subcellular localization of COX I and COX II in rat tissue sections, embedded in LR Gold resin, using monoclonal antibodies for these proteins. Immunofluorescence labeling of BS-C-1 monkey kidney cells with these antibodies showed… 

Subcellular localization of fumarase in mammalian cells and tissues

Evidence is provided that fumarase in mammalian cells/tissues is mainly localized in mitochondria and significant amounts of this protein are not present in the cytosol and these studies reveal that in certain tissues, in addition to mitochondria, this protein is also present at specific extramitochondrial sites.

Polymorphisms in mitochondrial genes encoding complex I subunits are maternal factors of voluntary alcohol consumption in the rat

Amo acid variations in four complex I subunits encoded in the mitochondrial genome may contribute to explain the differences between UChA and UChB rats in their capacity to reoxidize NADH and in their alcohol intake, suggesting that mitochondrial genes may constitute maternal factors of alcoholism.

Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria‐Associated Membrane Fractions from Transfected Cells and from Human Cytomegalovirus‐Infected Primary Fibroblasts

Two robust procedures were developed to efficiently isolate mitochondria, ER, and MAM fractions while providing the substantial protein yields from HCMV‐infected primary fibroblasts and from transfected HeLa cells.

Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria‐Associated Membrane and Detergent Resistant Membrane Fractions from Transfected Cells and from Human Cytomegalovirus‐Infected Primary Fibroblasts

This unit includes protocols for isolation of detergent resistant membranes from subcellular fractions as well as techniques that allow visualization of the mitochondrial network disruption that occurs in permissively infected cells by their optimal resolution in Percoll gradients.

"Mighty-chondrial" DNA repair for mitigation of cardiac injury: focus on "A novel mtDNA repair fusion protein attenuates maladaptive remodeling and preserves cardiac function in heart failure".

  • Qun ChenF. Salloum
  • Biology
    American journal of physiology. Heart and circulatory physiology
  • 2018
Oxidative stress has been widely accepted to play a critical role in ischemia-reperfusion injury and the progression of heart failure, and ETC defects have been incriminated as key sources of ROS generation in cardiac diseases.

Submitochondrial organization of human mitochondrial aspartyl-tRNA synthetase and its implication in LBSL disease

The aim of the PhD project was to contribute to the understanding of the link between mutations in the human mitochondrial aspartyl-tRNA synthetase (mt-AspRS) and LBSL disease, by studying the

Modulation of rat pituitary growth hormone by 670 nm light.

Brain metabolic DNA: recent evidence for a mitochondrial connection

Additional data demonstrate that BMD sequences are modified by learning, thus indicating that the modified synaptic activity allowing proper brain responses is encoded in learning BMD.

Mitochondrial DNA heteroplasmy in ovine fetuses and sheep cloned by somatic cell nuclear transfer

It is reported that the first cases of recipient oocyte/nuclear donor mtDNA heteroplasmy in SCNT-derived ovine clones are reported, demonstrating that there is no species-effect hindering ovine nucleus-donor mtDNA from being transmitted to the somatic clonal offspring.




The indicated presence of cytochrome‐c outside mitochondria in certain tissues under normal physiological conditions raises interesting questions concerning translocation mechanisms and the cellular functions of cy to chrome‐c.

Localization of Mitochondrial 60-kD Heat Shock Chaperonin Protein (Hsp60) in Pituitary Growth Hormone Secretory Granules and Pancreatic Zymogen Granules

Findings raise interesting questions concerning translocation mechanisms and the cellular roles of Hsp60, including the morphological localization results in pancreatic acinar cells.

Immunoelectron microscopy provides evidence for the presence of mitochondrial heat shock 10-kDa protein (chaperonin 10) in red blood cells and a variety of secretory granules

Similar to a number of other recently described mitochondrial proteins, Hsp10 is also found at a variety of specific extramitochondrial sites in normal rat tissue, which raises important questions as to how these mitochondrial proteins are translocated to other compartments and their possible function(s) at these sites.

Localization of a Mitochondrial Type of NADP-dependent Isocitrate Dehydrogenase in Kidney and Heart of Rat: An Immunocytochemical and Biochemical Study

Investigation of the subcellular localization of the mitochondrial type of NADP-dependent isocitrate dehydrogenase (ICD1) in rat was immunofluorescence and immunoelectron microscopy and by biochemical methods, including immunoblotting and Nycodenz gradient centrifugation, demonstrated that ICD1 was confined almost exclusively to mitochondria and nuclei of rat kidney and heart muscle.

Immunogold localization of mitochondrial aspartate aminotransferase in mitochondria and on the cell surface in normal rat tissues.

These findings support the previous identification of mAspAT as both a mitochondrial enzyme and a plasma membrane protein and suggest that in accordance with its established role in other cells and tissues, the surface-located mAsPAT in kidney and endothelial cells is involved in the fatty acid transport process.

Immunoelectron microscopy provides evidence that tumor necrosis factor receptor-associated protein 1 (TRAP-1) is a mitochondrial protein which also localizes at specific extramitochondrial sites.

The localization of this protein at specific extramitochondrial sites raises interesting and fundamental questions regarding the possible mechanisms by which these proteins are translocated to such sites.

Localization of P32 protein (gC1q-R) in mitochondria and at specific extramitochondrial locations in normal tissues

Evidence is provided that P32 (gC1q-R) is a mitochondrial protein that also localizes outside mitochondria in certain cells and tissues under normal physiological conditions.

Immunocytochemical localization of heat-shock protein 60-related protein in Beta-cell secretory granules and its altered distribution in non-obese diabetic mice

The altered distribution of the HSP60-related protein in prediabetic mice, together with the observation that immature secretory granules accumulate in these animals indicate that the presence of HSP 60-relatedprotein in secretorygranules might be associated with the secretory function of Beta cells.