Localization of mRNAs encoding Ca2+-inhibitable adenylyl cyclases along the renal tubule. Functional consequences for regulation of the cAMP content.

@article{Chabards1996LocalizationOM,
  title={Localization of mRNAs encoding Ca2+-inhibitable adenylyl cyclases along the renal tubule. Functional consequences for regulation of the cAMP content.},
  author={D. Chabard{\`e}s and Dmitri Firsov and Lotfi Aarab and A Clabecq and A. Bellanger and Sylvie Siaume-Perez and Jean-Marc Elalouf},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 32},
  pages={19264-71}
}
Expression of Ca2+-inhibitable types V and VI adenylyl cyclases was studied by reverse transcription-polymerase chain reaction in rat renal glomeruli and nephron segments isolated by microdissection. Quantitation of each mRNA was achieved using a mutant cRNA which differed from the wild type by substituting two bases to create a new restriction site in the corresponding cDNA. Type VI mRNA was present all along the nephron but was more abundant in distal than in proximal segments. The expression… CONTINUE READING