Localization of germline marker vasa homolog RNA to a single blastomere at early cleavage stages in the oriental river prawn Macrobrachium nipponense: evidence for germ cell specification by preformation.

@article{Qiu2013LocalizationOG,
  title={Localization of germline marker vasa homolog RNA to a single blastomere at early cleavage stages in the oriental river prawn Macrobrachium nipponense: evidence for germ cell specification by preformation.},
  author={G F Qiu and Yin Chen and Zheng Cui and Xiao-ling Zhu},
  journal={Gene},
  year={2013},
  volume={513 1},
  pages={53-62}
}
Germ cells are specified by the inheritance of maternal germline determinants (preformation mode) or inductive signals from somatic cells (epigenesis mode) during embryogenesis. However, the germline specification in decapod crustaceans is unclear so far. Using vasa homolog (MnVasa) as a germ cell marker, here we probed the early events of germline specification in the oriental river prawn Macrobrachium nipponense. Quantitative RT-PCR analysis of unfertilized eggs and embryos demonstrated that… CONTINUE READING
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