Localization of epithelial sodium channel (ENaC) and CFTR in the germinal epithelium of the testis, Sertoli cells, and spermatozoa

  title={Localization of epithelial sodium channel (ENaC) and CFTR in the germinal epithelium of the testis, Sertoli cells, and spermatozoa},
  author={Sachin Sharma and Aaron Hanukoglu and Israel Hanukoglu},
  journal={Journal of Molecular Histology},
Spermatogenesis starts within the seminiferous tubules of the testis by mitotic division of spermatogonia that produces spermatocytes. Meiotic division of these spermatocytes produces haploid spermatids that differentiate into spermatozoa. In this study, we examined the expression of ENaC and CFTR (a Cl− channel) in rat testicular sections using confocal microscopic immunofluorescence. The structural integrity of the seminiferous tubule sections was verified by precise phalloidin staining of… 

Mapping the sites of localization of epithelial sodium channel (ENaC) and CFTR in segments of the mammalian epididymis

To map the sites of localization of ion channels ENaC and CFTR along the length of the mouse and rat epididymis using confocal microscopic imaging, it is suggested that mutations in EN aC subunits may also be associated with male infertility.

Syntaxin binding protein 2 in sertoli cells regulates spermatogonial stem cell maintenance through directly interacting with connexin 43 in the testes of neonatal mice.

A novel regulatory mechanism in which the STXBP2/Cx43 complex is essential for the maintenance of Sertoli-germline interactions is demonstrated.

High‐resolution imaging of the actin cytoskeleton and epithelial sodium channel, CFTR, and aquaporin‐9 localization in the vas deferens

The localization of all three channels on the apical surface of the columnar epithelial cells provides clear evidence that these channels are involved concurrently in the regulation of fluid and electrolyte balance in the lumen of the vas deferens.

Identification and classification of epithelial cells in nephron segments by actin cytoskeleton patterns

This study highlights the power of high‐resolution confocal microscopy for identifying specific cell types using the simple probe of F‐actin‐binding phalloidin to identify whole kidney sections by confocal microscopic imaging of fluorescent phalloids.

A Review on the Role of Bicarbonate and Proton Transporters during Sperm Capacitation in Mammals

The involvement of Na+/H+ exchangers (NHE) belonging to the SLC9 family and/or voltage-gated proton channels (HVCN1) in sperm capacitation and the acrosome reaction is discussed.

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

Clarifying the mechanisms of signaling pathways in Sertoli cells on spermatogenesis provides new insights into the physiological functions of SERToli cells in male reproduction, and also serves as a pre-requisite to identify potential therapeutic targets in abnormal sperMatogenesis including testicular tumor and male infertility.

Epithelial sodium channel (ENaC) (version 2019.4) in the IUPHAR/BPS Guide to Pharmacology Database

ENaC function is also essential for the clearance of respiratory airways, transport of germ cells, fertilization, implantation and cell migration, and evidence has been provided that rare mutations in ENaC are associated with female infertility.

Experimental Investigation of Ki67, POU5F1, and ZBTB16 Expression in the Pig and Mouse Testicular Cells using Immunocytochemistry and RT-PCR

The nature of the SSC resulted after separation and culture was proved through measures, such as cluster growth of the colonies in the culture medium, Ki67 marker expression in the immunocytochemistry review which showed the duplication ability, and the morphological criteria observed by an electron microscope.



The Mammalian Blood-Testis Barrier: Its Biology and Regulation.

The biology and regulation of the mammalian blood-testis barrier is reviewed and research areas that should be expanded in future studies are highlighted to create a unique microenvironment for the completion of meiosis and the subsequent development of spermatids into spermatozoa via spermiogenesis.

Regulation of blood-testis barrier by actin binding proteins and protein kinases.

It is illustrated that the Sertoli cell BTB is regulated by actin-binding proteins (ABPs), likely supported by non-receptor protein kinases, to modulate the organization of actin microfilament bundles at the site.

Sertoli Cell Ectoplasmic Specializations in the Seminiferous Epithelium of the Testosterone-Suppressed Adult Rat1

It is concluded that the Sertoli cell ectoplasmic specialization structure is qualitatively normal in TE-treated rats, and thus the absence of this structure is unlikely to be the cause of round spermatid detachment.


In the present work, the cellular associations were defined by the stage of development of the spermatids, which themselves were characterized by their staining reaction to the “periodic acid-Schiff” technique (hereafter referred to as PAFSA).

Spermiation: The process of sperm release.

The causes of spermiation failure and their impact on sperm morphology and function are assessed in an effort to understand how this process may contribute to sperm count suppression during contraception and to phenotypes of male infertility.

Role of spermatogonia in the repair of the seminiferous epithelium following x-irradiation of the rat testis.

It was concluded that the type A0 were comparatively more resistant to x-irradiation than type A1 spermatogonia and thereby contributed to the reconstruction of the sperMatogonial population partially destroyed by irradiation.

Biology of the Sertoli Cell in the Fetal, Pubertal, and Adult Mammalian Testis.

Within this framework, this work provides an overview of the biology of the fetal, pubertal, and adult Sertoli cell, highlighting relevant cell biology studies that have expanded the understanding of mammalian spermatogenesis.

Tubular Fluid Secretion in the Seminiferous Epithelium: Ion Transporters and Aquaporins in Sertoli Cells

Deepening the knowledge on the mechanisms involved in the secretion, composition and regulation of SFT is essential and will be a major step in understanding the infertility associated with some pathological conditions.

Expression of epithelial sodium channel (ENaC) and CFTR in the human epidermis and epidermal appendages

The results reveal that ENaC is expressed strongly in all epidermal layers except stratum corneum, and also in the sebaceous glands, eccrine glands, arrector pili smooth muscle cells, and intra-dermal adipocytes, and co-localized with F-actin.

Role of the testis interstitial compartment in spermatogonial stem cell function.

Interstitial and peritubular cells are critical for SSC function and are an important underlying factor promoting male fertility.