Localization of cyclic adenosine 3',5'-monophosphate-responsive element (CRE)-binding proteins by southwestern histochemistry.

@article{Koji1994LocalizationOC,
  title={Localization of cyclic adenosine 3',5'-monophosphate-responsive element (CRE)-binding proteins by southwestern histochemistry.},
  author={Takehiko Koji and Kiyoshi Komuta and Masayuki Nozawa and Shuich Yamada and Paul K. Nakane},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1994},
  volume={42 10},
  pages={1399-405}
}
Regulation of gene transcription requires an interaction between specific segments of nuclear DNA and specific proteins. We describe a method to localize the specific DNA-binding proteins using haptenized double-stranded (ds) DNAs. To demonstrate this method, an oligodeoxynucleotide (oligo-DNA) with a consensus base sequence of cyclic adenosine monophosphate-responsive element (CRE) (TGACGTCA) with three TTA repeats at the 5' end was synthesized. Since the CRE sequence is palindromic, the oligo… CONTINUE READING

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