Localization of carbonic anhydrase in living osteoclasts with bodipy 558/568-modified acetazolamide, a thiadiazole carbonic anhydrase inhibitor.

@article{Brubaker1999LocalizationOC,
  title={Localization of carbonic anhydrase in living osteoclasts with bodipy 558/568-modified acetazolamide, a thiadiazole carbonic anhydrase inhibitor.},
  author={Kristen D. Brubaker and Feng Mao and Carol V. Gay},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1999},
  volume={47 4},
  pages={545-50}
}
We describe the synthesis of Bodipy 558/568-modified acetazolamide, a fluorescent inhibitor of carbonic anhydrase and its use to localize the enzyme in living cells. The modified acetazolamide, with its specific sulfonamide group intact, labeled cells at concentrations as low as 10(-9) M, with a minimal loading time of 5 min. The staining was decreased by… CONTINUE READING