Localization of antigenic sites at the amino-terminus of rinderpest virus N protein using deleted N mutants and monoclonal antibody.

@article{Choi2003LocalizationOA,
  title={Localization of antigenic sites at the amino-terminus of rinderpest virus N protein using deleted N mutants and monoclonal antibody.},
  author={Kang-Seuk Choi and Jin-Ju Nah and Young-Joon Ko and Shien-young Kang and Yi-seok Joo},
  journal={Journal of veterinary science},
  year={2003},
  volume={4 2},
  pages={167-73}
}
The nucleocapsid (N) protein of rinderpest virus (RPV) is highly conserved, immunogenic, and abundantly expressed during infection. Six antigenic sites (sites A, B, C, D, E and F), defined previously by a competitive binding assay using corresponding monoclonal antibodies (Mabs), have been further localized by immunoassays using deleted N mutants. Five different forms of RPV N protein, containing residues aa 1-79, aa 1-149, aa 1-421, aa 414-525 and aa 1-525, were expressed as glutathione S… CONTINUE READING