Localization of actin in Moloney murine leukemia virus by immunoelectron microscopy.

@article{Nermut1999LocalizationOA,
  title={Localization of actin in Moloney murine leukemia virus by immunoelectron microscopy.},
  author={Milan V. Nermut and K Wallengren and Jeanne Pager},
  journal={Virology},
  year={1999},
  volume={260 1},
  pages={23-34}
}
Immunoelectron microscopy was used to detect actin in wild-type (wt) Moloney murine leukemia virus (MoMuLV) and in virus-like particles (VLP) produced by recombinant Semliki Forest virus expressing only the MoMuLV gag polyprotein. Gold immunolabeling revealed the presence of actin on the surface of delipidized VLP and delipidized wt virus particles. Statistical evaluation of the number of colloidal gold particles per VLP revealed a large range of values and a prevalence of VLP with small… CONTINUE READING

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