Localization of actin in Chlamydomonas using antiactin and NBD-phallacidin.

  title={Localization of actin in Chlamydomonas using antiactin and NBD-phallacidin.},
  author={Patricia A. Detmers and Joan M. Carboni and J. Condeelis},
  journal={Cell motility},
  volume={5 5},
We have localized actin in gametes of Chlamydomonas reinhardi by two approaches: (1) indirect immunofluorescence with an affinity-purified antibody and (2) staining with NBD-phallacidin, a fluorescent reagent that binds only to F-actin [Barak et al, 1980, Proc Natl Acad Sci, 77:980-984]. Staining of either mating type "plus" (mt+) or "minus" (mt-) gametes with antiactin antibody resulted in similar fluorescent images: most of the actin was located peripherally along the lateral and posterior… CONTINUE READING