Localization of a wide-ranging panel of antigens in the rat retina by immunohistochemistry: comparison of Davidson's solution and formalin as fixatives.

@article{Chidlow2011LocalizationOA,
  title={Localization of a wide-ranging panel of antigens in the rat retina by immunohistochemistry: comparison of Davidson's solution and formalin as fixatives.},
  author={Glyn Chidlow and Mark Daymon and John P. M. Wood and Robert J. Casson},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2011},
  volume={59 10},
  pages={884-98}
}
The preferred fixative for whole eyes is Davidson's solution, which provides optimal tissue preservation while avoiding retinal detachment. Hitherto, the compatibility of Davidson's solution with immunohistochemistry has been largely untested. The goal of the present study was to compare the immunolabeling patterns of a wide-ranging panel of commercially available, previously validated antibodies in formalin- and Davidson's-fixed retinas. Immunohistochemistry was performed in normal pigmented… CONTINUE READING

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