Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast

@article{Klein1992LocalizationOR,
  title={Localization of RAP1 and topoisomerase II in nuclei and meiotic chromosomes of yeast},
  author={Franz Klein and Thierry Laroche and Maria Elena Cardenas and Johannes FX Hofmann and Dieter Schweizer and Susan M. Gasser},
  journal={The Journal of Cell Biology},
  year={1992},
  volume={117},
  pages={935 - 948}
}
Topoisomerase II (topoII) and RAP1 (Repressor Activator Protein 1) are two abundant nuclear proteins with proposed structural roles in the higher-order organization of chromosomes. Both proteins co-fractionate as components of nuclear scaffolds from vegetatively growing yeast cells, and both proteins are present as components of pachytene chromosome, co-fractionating with an insoluble subfraction of meiotic nuclei. Immunolocalization using antibodies specific for topoII shows staining of an… CONTINUE READING