Localization of HTLV-I tax proviral DNA in mononuclear cells.

  title={Localization of HTLV-I tax proviral DNA in mononuclear cells.},
  author={Dorothea Zucker-Franklin and Bette A. Pancake and Vesna Najfeld},
  journal={Blood cells, molecules & diseases},
  volume={31 1},
The tax sequence of HTLV-I is demonstrable in the skin and blood mononuclear cells of patients with mycosis fungoides, as well as in the mononuclear leukocytes of some healthy blood donors, but was not demonstrable when PCR/Southern analyses were carried out on preparations of high-molecular-weight genomic DNA. Therefore, it was postulated that tax DNA may not be integrated. To investigate this possibility fluorescence in situ hybridization was carried out on cells arrested in metaphase, using… CONTINUE READING