Localization of GFP-tagged concentrative nucleoside transporters in a renal polarized epithelial cell line.

@article{Mangravite2001LocalizationOG,
  title={Localization of GFP-tagged concentrative nucleoside transporters in a renal polarized epithelial cell line.},
  author={Lara M Mangravite and Joshua H. Lipschutz and Keith E. Mostov and Kathleen M. Giacomini},
  journal={American journal of physiology. Renal physiology},
  year={2001},
  volume={280 5},
  pages={F879-85}
}
Many nucleosides undergo active reabsorption within the kidney, probably via nucleoside transporters. To date, two concentrative nucleoside transporters have been cloned, the sodium-dependent purine-selective nucleoside transporter (SPNT) and concentrative nucleoside transporter 1 (CNT1). We report the stable expression of green fluorescence protein (GFP)-tagged SPNT and CNT1 in Madin-Darby canine kidney (MDCK) cells, a polarized renal epithelial line. We demonstrate that the GFP tag does not… CONTINUE READING
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