Localization and regulation of phospholipase D2 by ARF6.

  title={Localization and regulation of phospholipase D2 by ARF6.},
  author={Masami Hiroyama and John H. Exton},
  journal={Journal of cellular biochemistry},
  volume={95 1},
Phospholipase D (PLD) and ADP-ribosylation factor 6 (ARF6) have been implicated in vesicular trafficking and rearrangement of the actin cytoskeleton. We have explored the co-localization of rat PLD1b and rat PLD2 with wild type and mutant forms of ARF6 in HeLa cells and studied their activation by ARF6 and the role of the actin cytoskeleton. GFP-tagged PLD1 had a similar pattern to multivesicular and late endosomes and the trans-Golgi apparatus, but not to other organelles. When wild type or… CONTINUE READING