Localization and pharmacological characterization of voltage dependent calcium channels in cultured neocortical neurons

  title={Localization and pharmacological characterization of voltage dependent calcium channels in cultured neocortical neurons},
  author={Daniel B. Timmermann and Trine Meldgaard Lund and Bo Belhage and Arne Schousboe},
  journal={International Journal of Developmental Neuroscience},

Role of Astrocytes in Depolarization-Coupled Release of Glutamate in Cerebellar Cultures

The results support the notion of an important function of the astrocytes in the uptake of released glutamate, because dl-TBOA caused a large, apparent increase in the depolarization-coupled release of preloaded D-[3H]aspartate in the cocultures, compared to neuronal monocultures.

Transcriptomic Profiling of Ca2+ Transport Systems during the Formation of the Cerebral Cortex in Mice

A detailed view of the pattern of expression of the main actors participating in the import, export, and release of Ca2+.

Antiepileptic Drugs Targeting Cerebral Presynaptic Ion Channels Reduce Cerebral Excitability Decreasing Glutamate Release

The strategies that are used for investigating the effects of several compounds known for their anticonvulsant properties, including several of the most commonly used antiepileptic drugs of the first and second generations, as well as of the new potential antiePileptic drug, vinpocetine on cerebral presynaptic ionic channels are described.

'Pre-synaptic' vesicular glutamate release mechanisms in osteoblasts.

  • P. Bhangu
  • Biology, Medicine
    Journal of musculoskeletal & neuronal interactions
  • 2003
The overwhelming conclusion of this review is that by defining both the characteristics and regulatory control of this process, highlighting both similarities and differences between the CNS and bone may provide compelling evidence for the role of glutamate in bone cell function and physiology.



Role of calcium channel subtypes in calcium transients in hippocampal CA3 neurons

It is found that L-type calcium channels have a predominant role in somal calcium transients elicited by trains of strong stimuli applied to either the soma or the distal apical dendrite while class A calcium channels make a smaller contribution.

Three types of neuronal calcium channel with different calcium agonist sensitivity

Evidence is reported for the coexistence of three types of Ca channel in sensory neurones of the chick dorsal root ganglion and the dihydropyridine Ca agonist Bay K 8644 strongly increases the opening probability of L-, but not T- or N-type channels.

Calcium transients in dendrites of neocortical neurons evoked by single subthreshold excitatory postsynaptic potentials via low-voltage-activated calcium channels.

  • H. MarkramB. Sakmann
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1994
The results provide direct evidence that dendrites do not function as passive cables even at low-frequency synaptic activity; rather, a single subthreshold EPSP changes the dendritic membrane conductance by opening Ca2+ channels and generating a [Ca2+]i transient that may propagate towards the soma.

Pharmacological characterisation of voltage‐sensitive calcium channels and neurotransmitter release from mouse cerebellar granule cells in culture

Using subtype‐specific Ca‐channel blockers, the voltage‐sensitive Ca2+ currents as well as neurotransmitter release from cultured mouse cerebellar granule cells are characterised using the whole cell version of the patch clamp technique.

Clustering of L-type Ca2+ channels at the base of major dendrites in hippocampal pyramidal neurons

This work shows that L-type Ca2+channels, visualized using a monoclonal antibody, are located in the cell bodies and proximal dendrites of hippocampal pyramidal cells and are clustered in high density at the base of major dendrite.

A model for dendritic Ca2+ accumulation in hippocampal pyramidal neurons based on fluorescence imaging measurements.

Simulations of a biophysical model of intracellular Ca2+ concentration changes in hippocampal neurons injected with the Ca(2+)-sensitive indicator fura-2 support the hypotheses generated from experimental evidence regarding the differential distribution of voltage-gated Ca2- and Na+ channels in hippocampusal neurons and the resulting voltage- G2+ accumulation from action and synaptic potentials.

Dominant role of N-type Ca2+ channels in evoked release of norepinephrine from sympathetic neurons.

N-type calcium channels play a dominant role in the depolarization-evoked release of norepinephrine, and are involved in stimulus-secretion coupling.

Dendritic calcium transients evoked by single back‐propagating action potentials in rat neocortical pyramidal neurons.

The decay time course of the dendritic fluorescence transient was prolonged by the blockers of endoplasmic reticulum (ER) Ca(2+)‐ATPase, cyclopiazonic acid and thapsigargin, suggesting that uptake of Ca2+ into the ER in dendrites governs clearance of dendrite Ca2+.