Localization and characterization of phenamil-sensitive Na+ influx in isolated rainbow trout gill epithelial cells.

@article{Reid2003LocalizationAC,
  title={Localization and characterization of phenamil-sensitive Na+ influx in isolated rainbow trout gill epithelial cells.},
  author={Scott D. Reid and Guy S Hawkings and Fernando Esp{\'i}n G{\'a}lvez and Greg G. Goss},
  journal={The Journal of experimental biology},
  year={2003},
  volume={206 Pt 3},
  pages={551-9}
}
Percoll density-gradient separation, combined with peanut lectin agglutinin (PNA) binding and magnetic bead separation, was used to separate dispersed fish gill cells into sub-populations. Functional characterization of each of the sub-populations was performed to determine which displayed acid-activated phenamil- and bafilomycin-sensitive Na(+) uptake. Analysis of the mechanism(s) of (22)Na(+) influx was performed in control and acid-activated (addition of 10 mmoll(-1) proprionic acid) cells… CONTINUE READING
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