Localization and DNA sequence of a replication origin in the rhodopsin gene locus of Chinese hamster cells.

@article{Gale1992LocalizationAD,
  title={Localization and DNA sequence of a replication origin in the rhodopsin gene locus of Chinese hamster cells.},
  author={James M. Gale and R. A. Tobey and J. A. D'Anna},
  journal={Journal of molecular biology},
  year={1992},
  volume={224 2},
  pages={343-58}
}
A chromosomal origin of DNA replication has been localized within the single-copy rhodopsin gene locus in Chinese hamster (line CHO) cells using two methods. In the first method, single-copy segments were identified at 3 to 15 kb intervals within approximately 75 kb (kb = 10(3) bases) of cloned genomic DNA containing the early-replicating rhodopsin gene near its middle. The cloned single-copy segments were then used as hybridization probes to quantify the replication of their corresponding… CONTINUE READING