Localisation of N-acetylaspartate in oligodendrocytes/myelin

@article{Nordengen2013LocalisationON,
  title={Localisation of N-acetylaspartate in oligodendrocytes/myelin},
  author={Kaja Nordengen and Christoph Heuser and Johanne Egge Rinholm and Reuben Matalon and Vidar Gundersen},
  journal={Brain Structure and Function},
  year={2013},
  volume={220},
  pages={899-917}
}
The role of N-acetylaspartate in the brain is unclear. Here we used specific antibodies against N-acetylaspartate and immunocytochemistry of carbodiimide-fixed adult rodent brain to show that, besides staining of neuronal cell bodies in the grey matter, N-acetylaspartate labelling was present in oligodendrocytes/myelin in white matter tracts. Immunoelectron microscopy of the rat hippocampus showed that N-acetylaspartate was concentrated in the myelin. Also neuronal cell bodies and axons… Expand
Phenotype-Dependent Interactions between N-acetyl-L-Aspartate and Acetyl-CoA in Septal SN56 Cholinergic Cells Exposed to an Excess of Zinc.
TLDR
Investigation of whether zinc-excess may evoke differential effects on the NAA metabolism in neuronal cells with low and high expression of the cholinergic phenotype indicates that the acetyl-CoA synthesizing system in neurons constitutes functional unity with energy generating and NAA or acetylcholine pathways of its utilization, which are uniformly affected by neurotoxic conditions. Expand
Adapting brain metabolism to myelination and long‐range signal transduction
TLDR
The specific demands of oligodendrocytes during myelination and potential routes of metabolites between glial cells and myelinated axons are discussed and four specific metabolites are highlighted that contribute to the specific functions of white matter glia. Expand
Metabolic Changes in Synaptosomes in an Animal Model of Schizophrenia Revealed by 1H and 1H,13C NMR Spectroscopy
TLDR
A proof-of-concept application of one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy for analyzing the metabolic composition of synaptosomes is reported, demonstrating the feasibility of using NMR Spectroscopy to identify and quantify metabolites withinsynaptosomal fractions. Expand
The Current Challenges for Drug Discovery in CNS Remyelination
TLDR
An overview of how the field of in vitro models developed over recent years and recent biological findings about how oligodendrocytes mature after reactivation of their staminal niche are provided. Expand
Evaluation of neuron-glia integrity by in vivo proton magnetic resonance spectroscopy: Implications for psychiatric disorders
TLDR
Evidence is collected showing that glutamate/glutamine change is a good marker for impaired neuron-astrocyte integrity and that changes in N-acetylaspartate and lipid precursors reflect impaired myelination. Expand
Cerebral lactate metabolism and memory: Implications for Alzheimer's disease
TLDR
Production of lactate, via aerobic glycolysis, is beneficial for memory function during normal aging, yet is not explicitly required for spatial memory tasks, according to this research. Expand
Cortical N‐acetylaspartate concentrations are impacted in chronic stroke but do not relate to motor impairment: A magnetic resonance spectroscopy study
TLDR
Cortical MRS metabolites may have limited clinical utility as prospective biomarkers of upper‐extremity outcomes in chronic stroke, but may provide mechanistic insights into cellular processes of cortical adaptation to stroke. Expand
Magnetic resonance spectroscopy evidence for declining gliosis in MS patients treated with ocrelizumab versus interferon beta-1a
TLDR
Results from this exploratory study suggest that over 2 years, ocrelizumab reduces gliosis compared with interferon beta-1a, demonstrated by declining ml, and stable tCr and tCho levels. Expand
Identification of altered brain metabolites associated with TNAP activity in a mouse model of hypophosphatasia using untargeted NMR‐based metabolomics analysis
TLDR
These metabolites identify several biochemical processes that directly or indirectly involve TNAP function, in particular through the regulation of ecto‐nucleotide levels and of pyridoxal phosphate‐dependent enzymes, which may contribute to the neurodevelopmental and neurological phenotype of HPP. Expand
Global brain volume and N-acetyl-aspartate decline over seven decades of normal aging
TLDR
It is concluded that contrary to the hypothesis, atrophy was accompanied by WBNAA decline, and women's brains showed less atrophy than men's across the entire age range. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 75 REFERENCES
Differential distribution of N-acetylaspartylglutamate and N-acetylaspartate immunoreactivities in rat forebrain
TLDR
Although N-acetylaspartate-immunoreactivity was found in most neurons, it exhibited a great range of intensities between different neuronal types, and was observed in specific neuronal populations, including many groups thought to use GABA as a neurotransmitter. Expand
Expression of N-acetylaspartate and N-acetylaspartylglutamate in the nervous system.
TLDR
The water-soluble carbodiimide, EDAC (1-ethyl-3 [3-dimethylaminopropyl] carbodiIMide hydrochloride), is used for the immunohistochemical localization of NAAG and NAA, both of which are found primarily in neurons. Expand
N-Acetylaspartate in neuropsychiatric disorders
TLDR
Exogenous NAA is increasingly being exploited as a marker for functional and structural integrity of neurons in an expanding number of disorders, including Alzheimer's Disease and Huntington's Disease. Expand
Intraneuronal N‐acetylaspartate supplies acetyl groups for myelin lipid synthesis: evidence for myelin‐associated aspartoacylase
TLDR
It is demonstrated that within white matter, aspartoacylase is an integral component of the myelin sheath where it is ideally situated to produce acetyl groups for synthesis of myelin lipids. Expand
Immunocytochemical localization of N-acetyl-aspartate with monoclonal antibodies
TLDR
Immunocytochemical staining has shown that N-acetyl-aspartate-like immunoreactivity is localized in neurons, which are widely distributed throughout the brain. Expand
Enhanced carbodiimide fixation for immunohistochemistry: application to the comparative distributions of N-acetylaspartylglutamate and N-acetylaspartate immunoreactivities in rat brain.
TLDR
Perfusion protocols were tested for their ability to improve the immunohistochemical localization of two nervous system-specific molecules, NAAG and N-acetylaspartate, in the spinal cord, medulla, hippocampus, and cerebral cortex of the rat. Expand
The effect of N-acetyl-aspartyl-glutamate and N-acetyl-aspartate on white matter oligodendrocytes
TLDR
It is concluded that an action of NAAG on oligodendrocyte NMDA receptors is unlikely to be a major contributor to white matter damage in the leucodystrophies. Expand
In Vitro Expression of N‐Acetyl Aspartate by Oligodendrocytes
TLDR
The data presented here support the hypothesis that oligodendrocytes may express NAA in vivo and contribute to the NAA signal observed by in vivo 1H MRS. Expand
Extensive aspartoacylase expression in the rat central nervous system
Aspartoacylase (ASPA) catalyzes deacetylation of N‐acetylaspartate (NAA) to generate acetate and aspartate. Mutations in the gene for ASPA lead to reduced acetate availability in the CNS duringExpand
Immunohistochemical localization of N-acetylaspartate in rat brain.
TLDR
NAAG and NAA immunoreactivities were extensively colocalized in many brainstem areas, where NAAG containing neurons were more numerous than in forebrain structures. Expand
...
1
2
3
4
5
...