Local somatic hypermutation and class switch recombination in the nasal mucosa of allergic rhinitis patients.

@article{Coker2003LocalSH,
  title={Local somatic hypermutation and class switch recombination in the nasal mucosa of allergic rhinitis patients.},
  author={Heather A. Coker and Stephen R. Durham and Hannah J Gould},
  journal={Journal of immunology},
  year={2003},
  volume={171 10},
  pages={5602-10}
}
Immunoglobulin E is produced by nasal B cells in response to allergen. We have analyzed IgE V(H) region sequences expressed in the nasal mucosa of patients suffering from allergic rhinitis. V(H) region sequences were amplified by RT-PCR from IgE(+) B cells from nasal biopsies. In two of six patients, sequence analysis clearly demonstrated the presence of closely related IgE(+) B cell clones: cells displaying identical signature regions across CDR3/FWR4, indicating a common clonal ancestry, but… CONTINUE READING