Local sequence and sequencing depth dependent accuracy of RNA-seq reads

@inproceedings{Cai2017LocalSA,
  title={Local sequence and sequencing depth dependent accuracy of RNA-seq reads},
  author={Guoshuai Cai and Shoudan Liang and Xiaofeng Zheng and Feifei Xiao},
  booktitle={BMC Bioinformatics},
  year={2017}
}
Many biases and spurious effects are inherent in RNA-seq technology, resulting in a non-uniform distribution of sequencing read counts for each base position in a gene. Therefore, a base-level strategy is required to model the non-uniformity. Also, the properties of sequencing read counts can be leveraged to achieve a more precise estimation of the mean and… CONTINUE READING