Local mobility within villin 14T probed via heteronuclear relaxation measurements and a reduced spectral density mapping.

@article{Markus1996LocalMW,
  title={Local mobility within villin 14T probed via heteronuclear relaxation measurements and a reduced spectral density mapping.},
  author={Michelle A. Markus and Kwaku T. Dayie and P Matsudaira and Gerhard Wagner},
  journal={Biochemistry},
  year={1996},
  volume={35 6},
  pages={1722-32}
}
Villin 14T, a representative domain from the actin severing and bundling protein villin, binds calcium ions and actin monomers. To begin to understand the contributions of mobility to the villin-calcium and villin-actin interactions, relaxation rates for magnetization involving the amide nitrogens and protons have been measured for 15N-labeled villin 14T in solution. Although we have measured the complete set of rates required for a full spectral density map, difficulties in the accurate… CONTINUE READING