Local dynamics of DNA probed with optical absorption spectroscopy of bound ethidium bromide.

@article{Cupane1997LocalDO,
  title={Local dynamics of DNA probed with optical absorption spectroscopy of bound ethidium bromide.},
  author={Antonio Cupane and Carolina Bologna and Oscar E Rizzo and Eugenio Vitrano and Lorenzo Cordone},
  journal={Biophysical journal},
  year={1997},
  volume={73 2},
  pages={959-65}
}
We have studied the local dynamics of calf thymus double-helical DNA by means of an "optical labeling" technique. The study has been performed by measuring the visible absorption band of the cationic dye ethidium bromide, both free in solution and bound to DNA, in the temperature interval 360-30 K and in two different solvent conditions. The temperature… CONTINUE READING