Local depletion of DNA methylation identifies a repressive p53 regulatory region in the NEK2 promoter.

@article{Nabilsi2013LocalDO,
  title={Local depletion of DNA methylation identifies a repressive p53 regulatory region in the NEK2 promoter.},
  author={Nancy H Nabilsi and Daniel J. Ryder and Ashley C Peraza-Penton and Rosha Poudyal and David S. Loose and Michael P. Kladde},
  journal={The Journal of biological chemistry},
  year={2013},
  volume={288 50},
  pages={35940-51}
}
Genome-scale mapping suggests that the function of DNA methylation varies with genomic context beyond transcriptional repression. However, the use of DNA-demethylating agents (e.g. 5-aza-2'-deoxycytidine (5aza-dC)) to study epigenetic regulation often focuses on gene activation and ignores repression elicited by 5aza-dC. Here, we show that repression of NEK2, which encodes the never in mitosis A (NIMA)-related kinase, by 5aza-dC is context-specific as NEK2 transcript levels were reduced in… CONTINUE READING