Local delivery of molecules from a nanopipette for quantitative receptor mapping on live cells.

@article{Babakinejad2013LocalDO,
  title={Local delivery of molecules from a nanopipette for quantitative receptor mapping on live cells.},
  author={Babak Babakinejad and Peter J{\"o}nsson and Ainara L{\'o}pez C{\'o}rdoba and Paolo Actis and Pavel Novak and Yasufumi Takahashi and Andrew Shevchuk and Uma Anand and P. R. Vijai Anand and Anna Drews and Antonio V. Ferrer-Montiel and David Klenerman and Yuri E. Korchev},
  journal={Analytical chemistry},
  year={2013},
  volume={85 19},
  pages={9333-42}
}
Using nanopipettes to locally deliver molecules to the surface of living cells could potentially open up studies of biological processes down to the level of single molecules. However, in order to achieve precise and quantitative local delivery it is essential to be able to determine the amount and distribution of the molecules being delivered. In this work, we investigate how the size of the nanopipette, the magnitude of the applied pressure or voltage, which drives the delivery, and the… CONTINUE READING
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