Local cooperativity in the unfolding of an amyloidogenic variant of human lysozyme

@article{Canet2002LocalCI,
  title={Local cooperativity in the unfolding of an amyloidogenic variant of human lysozyme},
  author={Denis Canet and Alexander M. Last and Paula Tito and Margaret Sunde and Andrew Spencer and David B. Archer and Christina Redfield and Carol V Robinson and Christopher M. Dobson},
  journal={Nature Structural Biology},
  year={2002},
  volume={9},
  pages={308-315}
}
Hydrogen exchange experiments monitored by NMR and mass spectrometry reveal that the amyloidogenic D67H mutation in human lysozyme significantly reduces the stability of the β-domain and the adjacent C-helix in the native structure. In addition, mass spectrometric data reveal that transient unfolding of these regions occurs with a high degree of cooperativity. This behavior results in the occasional population of a partially structured intermediate in which the three α-helices that form the… CONTINUE READING

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