Local control of T2/3 transitional cell carcinoma of bladder is correlated to differences in DNA supercoiling: evidence for two discrete tumor populations.

@article{Vaughan1993LocalCO,
  title={Local control of T2/3 transitional cell carcinoma of bladder is correlated to differences in DNA supercoiling: evidence for two discrete tumor populations.},
  author={Andrew T. Vaughan and Paul N. Anderson and D. Michael A. Wallace and Ronald P. Beaney and Thomas H. Lynch},
  journal={Cancer research},
  year={1993},
  volume={53 10 Suppl},
  pages={2300-3}
}
Single cell tumor suspensions were prepared from biopsy and urine samples from 48 patients with muscle invasive transitional cell carcinoma of the bladder. Prior to therapy, samples were irradiated in vitro with the condensation of DNA supercoils measured by the light scattered within a flow cytometer. Six months after completing a course of radiotherapy, the in vitro data were correlated with the presence or absence of local disease. After 12-Gy irradiation, nucleoid extraction and staining… CONTINUE READING