Local calcium release in mammalian skeletal muscle.

  title={Local calcium release in mammalian skeletal muscle.},
  author={Natalia Shirokova and Jes{\'u}s Manuel Mart{\'i}nez Garc{\'i}a and Eduardo R{\'i}os},
  journal={The Journal of physiology},
  volume={512 ( Pt 2)},
1. Fluo-3 fluorescence associated with Ca2+ release was recorded with confocal microscopy in single muscle fibres mechanically dissected from fast twitch muscle of rats or frogs, voltage clamped in a two Vaseline-gap chamber. 2. Interventions that elicited Ca2+ sparks in frog skeletal muscle (low voltage depolarizations, application of caffeine) generated in rat fibres images consistent with substantial release from triadic regions, but devoid of resolvable discrete events. Ca2+ sparks were… CONTINUE READING