Local administration of WP9QY (W9) peptide promotes bone formation in a rat femur delayed-union model

  title={Local administration of WP9QY (W9) peptide promotes bone formation in a rat femur delayed-union model},
  author={Mikiya Sawa and Shigeyuki Wakitani and Naosuke Kamei and Shinji Kotaka and Nobuo Adachi and Mitsuo Ochi},
  journal={Journal of Bone and Mineral Metabolism},
The WP9QY peptide (W9) consists of nine amino acids. It binds to RANKL and blocks RANKL-induced increases in bone resorption and osteoclastogenesis. W9 has a unique effect on the coupling mechanism between osteoclasts and osteoblasts, which promotes bone formation while working to suppress bone resorption. In this study, with the aim of clinical application of W9 for fracture treatment, we aimed to clarify the bone repair-promoting effect of W9 when administered locally to a rat femur model of… 

Figures from this paper

Induction of chondrogenesis with a RANKL-binding peptide, WP9QY, in vitro and in vivo in a rabbit model
Treatment of OPG-deficient mice with WP9QY, a RANKL-binding peptide, recovers alveolar bone loss by suppressing osteoclastogenesis and enhancing osteoblastogenesis
W9 may be a useful drug to prevent alveolar bone loss in periodontitis by suppressing osteoclastogenesis and enhancing osteoblastogenesis by attenuating sclerostin expression in the alveolars of OPG–/–mice.
The W9 peptide inhibits osteoclastogenesis and osteoclast activity by downregulating osteoclast autophagy and promoting osteoclast apoptosis
The results showed that the W9 peptide could reduce osteoclastogenesis and osteooclast activity induced by RANKL, and these effects were partly due to the inhibition of osteocasts autophagy, which will benefit the development and application of new small molecule peptides for the treatment of bone resorption diseases.
Bifunctional Role of CrkL during Bone Remodeling
Data suggest that v-crk avian sarcoma virus CT10 oncogene homolog-like CrkL is involved in bone homeostasis, where it helps to regulate the complex interactions of the osteoblasts, osteoclasts, and their coupling signals.
Upregulation of Parkin Accelerates Osteoblastic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells and Bone Regeneration by Enhancing Autophagy and β-Catenin Signaling
Osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) is analyzed following upregulation or downregulation of Parkin to conclude that Parkin meditates osteoblastic differentiation of BMSCs via β-catenin and autophagy signaling.
Discovery of the RANKL/RANK/OPG system
  • H. Yasuda
  • Biology, Medicine
    Journal of Bone and Mineral Metabolism
  • 2021
Recent results showing the importance of RANKL on osteoblasts in regulation of osteogenesis and the role of RankingL-RANK dual signaling in coupling of bone resorption and formation are covered, including demonstration of RankL reverse signaling that was previously hypothesized.
Chimeric Peptides Quickly Modify the Surface of Personalized 3D Printing Titanium Implants to Promote Osseointegration.
3D-Ti implant surfaces were modified to reinforce osseointegration using chimeric peptides constructed by connecting W9, RP1P, and minTBP-1 directly or via (GP)4, respectively, which promote osseointedegration by promoting angiogenesis and osteogenesis.
New insights into the role of Methionine 199 in the function of Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL) and bone homeostasis
................................................................................................................ ii ACKNOWLEDGEMENTS


Stimulation of Bone Formation in Cortical Bone of Mice Treated with a Receptor Activator of Nuclear Factor-κB Ligand (RANKL)-binding Peptide That Possesses Osteoclastogenesis Inhibitory Activity
The findings suggest that this peptide may be useful for the treatment of bone diseases, and W9 achieves its bone anabolic activity through RANKL on osteoblasts accompanied by production of several autocrine factors.
Parathyroid hormone stimulates the bone apposition rate independently of its resorptive action: differential effects of intermittent and continuous administration.
The results suggest that the resorptive effects of bPTH-(1-84) can be separated from the effects of the hormone on the apposition rate, and also found an increase in both formation and resorption surfaces and a net decrease in the trabecular bone volume in this group.
Prevention of atrophic nonunion development by recombinant human bone morphogenetic protein‐7
Effects of local administration of GH and IGF-1 on longitudinal bone growth in rats.
It is confirmed and extends earlier investigations, showing that local injection of GH at the site of the epiphysial growth plate stimulates unilateral bone growth and that local administration of IGF-1 stimulates longitudinal bone growth.
A novel quercetin analogue from a medicinal plant promotes peak bone mass achievement and bone healing after injury and exerts an anabolic effect on osteoporotic bone: The role of aryl hydrocarbon receptor as a mediator of osteogenic action
  • K. Sharan, J. Mishra, N. Chattopadhyay
  • Biology, Medicine
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
  • 2011
The data suggest that GTDF stimulates osteoblast growth and differentiation via the AhR and promotes modeling‐directed bone accrual, accelerates bone healing after injury, and exerts anabolic effects on osteopenic rats likely by a direct stimulatory effect on osteoprogenitors.
Novel regulators of bone formation: molecular clones and activities.
Human complementary DNA clones corresponding to three polypeptides present in this BMP preparation have been isolated, and expression of the recombinant human proteins have been obtained, and each appears to be independently capable of inducing the formation of cartilage in vivo.
Stimulation of bone formation in vitro and in rodents by statins.
It is shown that the statins, drugs widely used for lowering serum cholesterol, also enhance new bone formation in vitro and in rodents, and may have therapeutic applications for the treatment of osteoporosis.
LDL Receptor-Related Protein 5 (LRP5) Affects Bone Accrual and Eye Development