Local RNA structural changes induced by crystallization are revealed by SHAPE.

  title={Local RNA structural changes induced by crystallization are revealed by SHAPE.},
  author={Quentin Vicens and Anne R. Gooding and Alain Laederach and Thomas R Cech},
  volume={13 4},
We present a simple approach to locate sites that undergo conformational changes upon crystallization by comparative structural mapping of the same RNA in three different environments. As a proof of principle, we probed the readily crystallized P4-P6DeltaC209 domain from the Tetrahymena thermophila group I intron in a native solution, in a solution mimicking the crystallization drop, and in crystals. We chose the selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) chemistry… CONTINUE READING


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