Loading of oxidizable transmitters into secretory vesicles permits carbon-fiber amperometry.

@article{Kim2000LoadingOO,
  title={Loading of oxidizable transmitters into secretory vesicles permits carbon-fiber amperometry.},
  author={Kyong Tae Kim and Dr Sock Huang Koh and Bertil Hille},
  journal={The Journal of neuroscience : the official journal of the Society for Neuroscience},
  year={2000},
  volume={20 20},
  pages={RC101}
}
Carbon-fiber amperometry detects oxidizable molecules released by exocytosis. We extended this electrochemical technique to cells that do not normally secrete oxidizable transmitters. We incubated AtT-20 cells, pituitary gonadotropes, cultured cerebellar granule cells, and yeast with high concentrations of dopamine (DA) and observed spontaneous and evoked quantal release of DA by amperometry. The rate of detectable spontaneous amperometric events was used as a measure of loading in AtT-20 cells… CONTINUE READING