Loading of Oxidizable Transmitters into Secretory Vesicles Permits Carbon-Fiber Amperometry

  title={Loading of Oxidizable Transmitters into Secretory Vesicles Permits Carbon-Fiber Amperometry},
  author={K. T. Kim and D. Koh and B. Hille},
  journal={The Journal of Neuroscience},
  pages={RC101 - RC101}
  • K. T. Kim, D. Koh, B. Hille
  • Published 2000
  • Medicine, Biology
  • The Journal of Neuroscience
  • Carbon-fiber amperometry detects oxidizable molecules released by exocytosis. We extended this electrochemical technique to cells that do not normally secrete oxidizable transmitters. We incubated AtT-20 cells, pituitary gonadotropes, cultured cerebellar granule cells, and yeast with high concentrations of dopamine (DA) and observed spontaneous and evoked quantal release of DA by amperometry. The rate of detectable spontaneous amperometric events was used as a measure of loading in AtT-20 cells… CONTINUE READING
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